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大肠杆菌载体 E.coli Vector

α-HL M113G 溶血素表达质粒

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α-HL M113G 溶血素表达质粒

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Description The plasmid α-HL M113G is designed for the preparation of the heptameric transmembrane protein alpha-hemolysin (α-HL) M113G by prokaryotic expression and to prove that methionine at site 113 is the key amino acid for gold ion binding. A H6 tag is designed for the followup purification using nickel affinity chromatography. Since 1996, α-HL WT along with its mutants have been widely investigated as single-molecule nanopore sensors, suitable for sensing a variety of analyte such as ionic species, small molecules, macromolecules and biomacromolecules.
Publication Cao, J., Jia, W., Zhang, J. et al. Giant single molecule chemistry events observed from a tetrachloroaurate(III) embedded Mycobacterium smegmatis porin A nanopore. Nat Commun 10, 5668 (2019) doi:10.1038/s41467-019-13677-2

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