产品介绍

DescriptionThe plasmid ClyA-AS is designed for the preparation of a mutant Cytolysin A (ClyA). ClyA is a cytolytic a-helical toxin which results haemolytic phenotype of several Escherichia coli and Salmonella enterica strains. The dodecameric ClyA is cylinder-shaped with a wide inner diameter of 70 A. The large inner diameter makes it capable for the detection of macromolecules, such as dsDNA and proteins. ClyA-AS, which is a mutant of ClyA, is engineered to avoid the effect of the negatively charged residues in the inner wall. It has been used to monitor the binding process of ligands to internalized proteins. (Soskine M, Biesemans A, Maglia G. Single-molecule analyte recognition with ClyA nanopores equipped with internal protein adaptors[J]. Journal of the American Chemical Society, 2015, 137(17): 5793-5797.) It is also employed as a nanoreactor to report the conformational changes of native proteins. ( Van Meervelt V, Soskine M, Singh S, et al. Real-time conformational changes and controlled orientation of native proteins inside a protein nanoreactor[J]. Journal of the American Chemical Society, 2017, 139(51): 18640-18646.).PublicationSingle-Molecule Analyte Recognition with ClyA Nanopores Equipped with Internal Protein Adaptors

基本信息