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LOCUS       pAG36        6123 bp    DNA   CIRCULAR SYN        19-JUN-2013
DEFINITION  
ACCESSION   P30110
KEYWORDS    .
SOURCE      Unknown.
ORGANISM  Unknown
Unclassified.
REFERENCE   1  (bases 1 to 6123)
AUTHORS   Self
JOURNAL   Unpublished.
COMMENT     Goldstein, A.L., and McCusker, J.H. 1999: Three new dominant 
drug resistance cassettes for gene disruption in Saccharomyces cerevisiae. 
Yeast 15, 1541-15
COMMENT     SECNOTES|Goldstein Sequenz wie folgt verändert (nach 
Sequenzierung, 
Nummerierung PK Sequenz)
Pos. zw. 2337 und 2338 CGC entfernt
Pos. 2474 C nach G
Pos. 2574 C nach G
Pos. 2575 G nach C
Pos. 2577 C nach G
Pos. 2588 T nach CPos. 2665 hier stimmt GO T in PK G nach T
nicht sequenziert
Pos. 3249 G nach A
Pos. zw 3335 und 3336 TAC entfernt
FEATURES             Location/Qualifiers
CDS             411..1214
/gene="URA3"
/product="URA3"
/SECDrawAs="Gene"
misc_feature    complement (2064..2303)
/gene="tAgTEF"
/product="terminator Ashbya gossypii TEF"
/SECDrawAs="Region"
CDS             complement (2304..2876)
/gene="nat1"
/product="Nourseothricin acetyltransferase"
/SECDrawAs="Gene"
misc_feature    complement (2877..3255)
/gene="pAgTEF"
/product="promoter Ashbya gossypii"
/SECDrawAs="Region"
misc_feature    3772..3867
/gene="ori"
/SECDrawAs="Region"
CDS             complement (4549..5409)
/gene="AmpR"
/product="AmpR"
/SECDrawAs="Gene"
misc_feature    5551..5668
/gene="CEN6"
/SECDrawAs="Region"
misc_feature    5681..6054
/gene="ARSH4"
/SECDrawAs="Region"
BASE COUNT     1630 a   1408 c   1536 g   1549 t
ORIGIN      
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag
421 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata
481 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat
541 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata
601 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt
661 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc
721 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg
781 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac
841 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat
901 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg
961 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg
1021 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg
1081 tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg
1141 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg
1201 gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt
1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta
1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
1441 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
1501 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
1561 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta1621 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
1681 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
1741 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg
1801 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
1861 ctattacgcc agctggcgaa ggggggatgt gctgcaaggc gattaagttg ggtaacgcca
1921 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
1981 tagggcgaat tggagctcca ccgcggtggc ggccgcatag gccactagtg gatctgatat
2041 catcgatgaa ttcgagctcg ttttcgacac tggatggcgg cgttagtatc gaatcgacag
2101 cagtatagcg accagcattc acatacgatt gacgcatgat attactttct gcgcacttaa
2161 cttcgcatct gggcagatga tgtcgaggcg aaaaaaaata taaatcacgc taacatttga
2221 ttaaaataga acaactacaa tataaaaaaa ctatacaaat gacaagttct tgaaaacaag
2281 aatcttttta ttgtcagtac tgattagggg cagggcatgc tcatgtagag cgcctgctcg
2341 ccgtccgagg cggtgccgtc gtacagggcg gtgtccaggc cgcagagggt gaaccccatc
2401 cgccggtacg cgtggatcgc cggtgcgttg acgttggtga cctccagcca gaggtgcccg
2461 gcgccccgct cgcgggcgaa ctccgtcgcg agccccatca acgcgcgccc gaccccgtgc
2521 ccccggtgct ccggggcgac ctcgatgtcc tcgacggtca gccggcggtt ccagccggag
2581 tacgagacga ccacgaagcc cgccaggtcg ccgtcgtccc cgtacgcgac gaacgtccgg
2641 gagtccgggt cgccgtcctc cccgtcgtcc gattcgtcgt ccgattcgtc gtcggggaac
2701 accttggtca ggggcgggtc caccggcacc tcccgcaggg tgaagccgtc cccggtggcg
2761 gtgacgcgga agacggtgtc ggtggtgaag gacccatcca gtgcctcgat ggcctcggcg
2821 tcccccggga cactggtgcg gtaccggtaa gccgtgtcgt caagagtggt acccatggtt
2881 gtttatgttc ggatgtgatg tgagaactgt atcctagcaa gattttaaaa ggaagtatat
2941 gaaagaagaa cctcagtggc aaatcctaac cttttatatt tctctacagg ggcgcggcgt
3001 ggggacaatt caacgcgtct gtgaggggag cgtttccctg ctcgcaggtc tgcagcgagg
3061 agccgtaatt tttgcttcgc gccgtgcggc catcaaaatg tatggatgca aatgattata
3121 catggggatg tatgggctaa atgtacgggc gacagtcaca tcatgcccct gagctgcgca
3181 cgtcaagact gtcaaggagg gtattctggg cctccatgtc gctggccggg tgacccggcg
3241 gggacaaggc aagctaaaca gatctggcgc gccttaatta acccggggat ccgtcgacct
3301 gcagcgtacg aagcttcagc tggcggccgc tctagccagc ttttgttccc tttagtgagg
3361 gttaattccg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
3421 gctcacaatt ccacacaaca taggagccgg aagcataaag tgtaaagcct ggggtgccta
3481 atgagtgagg taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
3601 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
3661 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
3721 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
3781 gctggcgttt ttccataggc tcggcccccc tgacgagcat cacaaaaatc gacgctcaag
3841 tcagaggtgg cgaaacccga caggactata aagataccag gcgttccccc ctggaagctc
3901 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
3961 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt
4021 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
4081 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
4141 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
4201 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
4261 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
4321 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
4381 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
4441 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
4501 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
4561 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
4621 gcccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
4681 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
4741 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
4801 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
4861 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
4921 ccaacgatca aggcgagtta catgatcccc catgttgtga aaaaaagcgg ttagctcctt
4981 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
5041 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
5101 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
5161 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
5221 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
5281 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
5341 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg5401 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
5461 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
5521 tccccgaaaa gtgccacctg ggtccttttc atcacgtgct ataaaaataa ttataattta
5581 aattttttaa tataaatata taaattaaaa atagaaagta aaaaaagaaa ttaaagaaaa
5641 aatagttttt gttttccgaa gatgtaaaag actctagggg gatcgccaac aaatactacc
5701 ttttatcttg ctcttcctgc tctcaggtat taatgccgaa ttgtttcatc ttgtctgtgt
5761 agaagaccac acacgaaaat cctgtgattt tacattttac ttatcgttaa tcgaatgtat
5821 atctatttaa tctgcttttc ttgtctaata aatatatatg taaagtacgc tttttgttga
5881 aattttttaa acctttgttt attttttttt cttcattccg taactcttct accttcttta
5941 tttactttct aaaatccaaa tacaaaacat aaaaataaat aaacacagag taaattccca
6001 aattattcca tcattaaaag atacgaggcg cgtgtaagtt acaggcaagc gatccgtcct
6061 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
6121 gtc
//

基本信息