产品介绍

基因型：F- λ- IN(rrnD-rrnE)1 rph-1 ΔlpxM ΔwecA-wzzE(gne)

This host strain is a derivative of the Raptor strain and can be used 

for bioengineering where purified products with reduced endotoxicity 

are desirable. Enterobacterial common antigen is no longer produced 

and a UDP N-acetylglucose epimerase has been chromosomally integrated 

increasing the availability of UDP-GalNAc.
The genes wecA-wzzE in the ECA synthesis locus were replaced with gne, 

UDP N-acetylglucose epimerase from the Campylobacter jejuni strain 81116,
which was inserted under control of the native, constitutive wecA promoter,

in the Raptor background using CRISPR technology.
wzzE encodes the enterobacterial common antigen chain length regulator 

which was removed to prevent any interference with recombinant polysaccharide 

production.

基本信息