产品介绍

Name: YEp24

DSM No.: 6185 dsm-6185 dsm6185 dsm 6185 YEp24

Other collection no.or WDCM no.: ATCC 37051, CCRC 41112

History: <- CCRC <- New England Bio-Labs <- ATCC

Mol. weight: 7.769 kb

Marker: Apr, ura 3+

Remarks: Yeast episomal plasmid with numerous unique restriction sites. Cloning vehicle for introduction of constructs into S. cerevisiae at a high copy number. Can replicate in both E. coli (due to pBR322 replication origin) and S. cerevisiae (due to the replication determinant of the yeast 2 micron circle plasmid). Apr is the selectable marker in E. coli, the URA3 gene of the yeast strain +D4 usable as selective marker in yeast. The promoter of the Tcr gene of pBR322 is separated by the URA3 sequence.

Distributed in: Escherichia coli K12 HB101

Cultivation conditions: Medium 381, 37°C

基本信息