|
Vector
|
Primer(F)
|
Primer(R)
|
抗性
|
载体大小(bp)
|
|
EZ-T
|
M13-47
|
RV-M
|
Amp+
|
2984
|
|
LZRS-RfA
|
LZRS-F
|
LZRS-R
|
|
11100
|
|
p3xFLAG CMV14
|
CMV30(834bp)
|
CMV24(1151bp)
|
|
6310
|
|
p3XFlag-myc-CMV-24
|
CMV-F(852bp)/PCEP-F(896bp)
|
R(hGH polyA)(1217bp)
|
|
4752
|
|
pAAV-MCS
|
F(β-globin)(1192bp)
|
R(hGH polyA)(1521bp)
|
|
4650
|
|
pAc5.1/V5-His A
|
Ac5-Profor(2525bp)
|
BGH(2768bp)/EBVrev(2844bp)
|
Amp+
|
5375
|
|
pAc5.1/V5-His B
|
Ac5-Profor(2525bp)
|
BGH(2772bp)/EBVrev(2848bp)
|
Amp+
|
5379
|
|
pAc5.1/V5-His C
|
Ac5-Profor(2525bp)
|
BGH(2764bp)/EBVrev(2840bp)
|
Amp+
|
5371
|
|
pAcGP67_A
|
PH-Profor(4016bp)
|
pAcGP67rv(4329bp)
|
Amp+
|
9761
|
|
pAcGP67_B
|
PH-Profor(4016bp)
|
pAcGP67rv(4329bp)
|
Amp+
|
9761
|
|
pAcGP67_C
|
PH-Profor(4016bp)
|
pAcGP67rv(4329bp)
|
Amp+
|
9761
|
|
pACT
|
T7(1061bp)
|
T3(1420bp)
|
Amp+
|
5566
|
|
pACT2
|
5'AD(同GAL4AD)(5134bp)
|
3'AD(4939bp)/pGAL4-Adrv(4964bp)(pGAL4-Adrv离MCS少于20bp，优先用3'AD)
|
Amp+
|
8117
|
|
PACYCDuet-1(MCSⅠ)
|
DuetUP1(3949bp)
|
DuetDOWN1(209bp)
|
Chl+
|
4008
|
|
PACYCDuet-1(MCSⅡ)
|
DuetUP2(189bp)
|
T7ter(468bp)
|
Chl+
|
4008
|
|
pAdTrack
|
pEGFP-N-3’(同EGFP-Nrev)(1294bp)
|
CMV-F(1433bp)
|
Amp+/Kan+
|
8111
|
|
pAdTrack-CMV
|
pEGFP-N-3’(同EGFP-Nrev)(1272bp)
|
EBVrev(1982bp)
|
Kan+
|
9010
|
|
PAO815
|
5'AOX
|
3'AOX
|
|
7700
|
|
pAS2-1
|
5'BD(同GAL4BD)(5894bp)
|
3'BD(6112bp)/pGAL4-BDrv(6046bp)(优先用3'BD)
|
Amp+
|
8392
|
|
pB42AD
|
pB42ADF(787bp)
|
pB42ADR(924bp)
|
Amp+
|
6449
|
|
PBABE-IRES-Egfp
|
pLXSN-F(同pLXSNfw)(1027bp)/PBABE5'(1063bp)/pBMN5'(1416bp)
|
无反向引物
|
|
5944
|
|
pBabe-puro
|
pBabe5'(1318bp)
|
pBabe3'(1429bp)
|
|
5169
|
|
pBACPAK8
|
BAC1(1181bp)/PH-Profor(1174bp)/BaculoVfor(1211bp)
|
BAC2(1374bp)/BaculoVrev(1375bp)
|
Amp+
|
5538
|
|
pBAD/His A
|
pBADfw(211bp)/Xpressfor(375bp)(优先用pBADfw)
|
pBADrv(514bp)
|
|
4102
|
|
pBBR1MCS
|
M13F(3161bp)/T7(3188bp)/M13-47(3135bp)
|
M13R(3390bp)/T3(3352bp)/RV-M(3409bp)
|
Amp+
|
4707
|
|
pBI121
|
35S(5735bp)
|
无
|
|
14758
|
|
pBK-CMV
|
T7(975bp)/M13F(951bp)
|
T3(1159bp)/M13R(1196bp)
|
Amp+
|
4518
|
|
pBluescript II KS (-)
|
M13F(599bp)/T7(626bp)
|
T3(792bp)/M13R(829bp)
|
|
2961
|
|
pBluescript II KS (+)
|
M13F(599bp)/T7(626bp)
|
T3(792bp)/M13R(829bp)
|
|
2961
|
|
pBluescript II SK (-)
|
M13F(599bp)/T7(626bp)
|
T3(792bp)/M13R(829bp)
|
|
2961
|
|
pBlueScript II SK(+)
|
M13F(599bp)/T7(626bp)
|
T3(792bp)/M13R(829bp)
|
|
2961
|
|
pBluescript KS (-)
|
M13F(602bp)/T7(626bp)
|
T3(790bp)/M13R(828bp)
|
|
2958
|
|
pBluescript KS (+)
|
M13F(602bp)/T7(626bp)
|
T3(790bp)/M13R(828bp)
|
|
2958
|
|
pBluescript SK (-)
|
M13F(602bp)/T7(626bp)
|
T3(790bp)/M13R(828bp)
|
|
2958
|
|
pBluescript SK (+)
|
M13F(602bp)/T7(626bp)
|
T3(790bp)/M13R(828bp)
|
|
2958
|
|
pBSN-T
|
T7
|
T3
|
Amp+
|
|
|
pBudCE4.1(MCSI)
|
CMV-Profor(552)/T7(638)
|
c-mycrev(742bp)/EBVrev(842bp)
|
|
4595
|
|
pBudCE4.2(MCSⅡ)
|
PEF-F(2999)
|
BGH(3235)
|
|
4595
|
|
pBV220
|
PBV220F(3592bp)
|
PBV220R(93bp)
|
|
3667
|
|
pCAMBIA1300
|
P1 (11472bp)
|
P2 (2728bp)
|
|
11566
|
|
pCAMBIA1301
|
P1 (10935bp)
|
P2 (11149bp)
|
|
11849
|
|
pCAMBIA1304
|
P1(11454bp)
|
P2(11668bp)
|
|
12361
|
|
pCAMBIA2300
|
M13F(8418bp)/M13R(-48) (8313bp)
|
M13F(-47)(8461bp)/ M13R(8349bp)
|
|
8742
|
|
pCANTAB_5E
|
pCANTAB5-S1(2265bp)/pCANTAB5-R1(2222bp)
|
pCANTAB5-S6(2471bp)/pCANTAB5-R2(2504bp)
|
|
4517
|
|
pCAT3-enhancer
|
RVP3(4215bp)
|
无
|
Amp+
|
4273
|
|
pCDFDuet
|
T7(214bp)
|
T7ter(467bp)
|
|
3781
|
|
pCDH-CMV-MCS-EF1-Puro
|
CMV-F(2164bp)/PCEP-F(2208bp)/pLNCX-F(2210bp)
|
无
|
|
7384
|
|
pcDNA1.1
|
CMV-F(2076bp)/T7(2170bp)
|
无
|
|
4000
|
|
pcDNA2.1
|
M13F/T7
|
M13R/SP6
|
|
3013
|
|
pcDNA3.0
|
pcDNA3fw(841bp)/CMV-Profor(777bp)/T7(863bp)
|
SP6(1016bp)/BGH(1053bp)
|
|
5446
|
|
pcDNA3.1 (-)
|
T7(863bp)/CMV-Profor(777bp)/pcDNA3fw(841bp)
|
BGH(1039bp)
|
|
5428
|
|
pcDNA3.1 (+)
|
T7(863bp)/CMV-Profor(777bp)/pcDNA3fw(841bp)
|
BGH(1039bp)
|
|
5428
|
|
pcDNA3.1/His A
|
T7(863bp)/CMV-Profor(777bp)
|
BGH(1122bp)
|
Amp+
|
5514
|
|
pcDNA3.1/His B
|
T7(863bp)/CMV-Profor(777bp)
|
BGH(1122bp)
|
Amp+
|
5514
|
|
pcDNA3.1/His C
|
T7(863bp)/CMV-Profor(777bp)
|
BGH(1122bp)
|
Amp+
|
5514
|
|
pcDNA3.1/myc-His A
|
T7(863bp)/CMV-Profor(777bp)
|
BGH(1099bp)/c-mycrev(1020bp)
|
Amp+
|
5494
|
|
pcDNA3.1/myc-His B
|
T7(863bp)/CMV-Profor(777bp)
|
BGH(1099bp)/c-mycrev(1020bp)
|
Amp+
|
5494
|
|
pcDNA3.1/myc-His C
|
T7(863bp)/CMV-Profor(777bp)
|
BGH(1099bp)/c-mycrev(1020bp)
|
Amp+
|
5494
|
|
pcDNA3.1/V5-His A
|
T7(863bp)/CMV-Profor(777bp)
|
BGH(1108bp)/V5rev(1035bp)
|
Amp+
|
5502
|
|
pcDNA3.1/V5-His B
|
T7(863bp)/CMV-Profor(777bp)
|
BGH(1108bp)/V5rev(1035bp)
|
Amp+
|
5502
|
|
pcDNA3.1/V5-His C
|
T7(863bp)/CMV-Profor(777bp)
|
BGH(1108bp)/V5rev(1035bp)
|
Amp+
|
5502
|
|
pcDNA3.1/Zeo(-)
|
T7(863bp)/CMV-F(769bp)
|
BGH(1038bp)
|
Amp+
|
5014
|
|
pcDNA3.1/Zeo(+)
|
T7(863bp)/CMV-F(769bp)
|
BGH(1039bp)
|
Amp+
|
5015
|
|
pcDNA3.3/TOPOTA
|
CMV-F(584bp)/PCEP-F(628bp)/pLNCX-F(630bp)
|
无
|
Amp+
|
5407
|
|
pcDNA4/HisMax A
|
Xpressfor(1119bp)
|
BGH(1282bp)
|
Amp+
|
5258
|
|
pcDNA4/HisMax B
|
Xpressfor(1119bp)
|
BGH(1282bp)
|
Amp+
|
5258
|
|
pcDNA4/HisMax C
|
Xpressfor(1119bp)
|
BGH(1282bp)
|
Amp+
|
5258
|
|
pcDNA4/HisMax-TOPO
|
Xpressfor(1119bp)
|
BGH(1302bp)
|
Amp+
|
5274
|
|
pcDNA4/HisMax-TOPO/lacZ
|
Xpressfor(1119bp)
|
BGH(4482bp)
|
Amp+
|
8354
|
|
pcDNA4/myc-His A
|
CMV-Profor(777bp)/T7(863bp)
|
BGH(1103bp)/ c-mycrev(1024bp)
|
Amp+
|
5079
|
|
pcDNA4/myc-His B
|
CMV-Profor(777bp)/T7(863bp)
|
BGH(1103bp)/ c-mycrev(1024bp)
|
Amp+
|
5079
|
|
pcDNA4/myc-His C
|
CMV-Profor(777bp)/T7(863bp)
|
BGH(1103bp)/ c-mycrev(1024bp)
|
Amp+
|
5079
|
|
pcDNA4/TO
|
TREfor(908bp)/CMV-Profor(777bp)
|
BGH(1106bp)
|
Amp+
|
5078
|
|
pcDNA4/V5-His A
|
CMV-Profor(777bp)/T7(863bp)
|
BGH(1117bp)/V5rev(1044bp)
|
Amp+
|
5092
|
|
pcDNA4/V5-His B
|
CMV-Profor(777bp)/T7(863bp)
|
BGH(1117bp)/V5rev(1044bp)
|
Amp+
|
5092
|
|
pcDNA4/V5-His C
|
CMV-Profor(777bp)/T7(863bp)
|
BGH(1117bp)/V5rev(1044bp)
|
Amp+
|
5092
|
|
pcDNA5/FRT
|
CMV-Profor(777bp)/T7(863bp)
|
BGH(1039bp)
|
Amp+
|
5069
|
|
pcDNA6/myc-His A
|
CMV-Profor(777bp)/T7(863bp)
|
BGH(1099bp)/c-mycrev(1020bp)
|
Amp+
|
5126
|
|
pcDNA6/myc-His B
|
CMV-Profor(777bp)/T7(863bp)
|
BGH(1099bp)/c-mycrev(1020bp)
|
Amp+
|
5126
|
|
pcDNA6/myc-His C
|
CMV-Profor(777bp)/T7(863bp)
|
BGH(1099bp)/c-mycrev(1020bp)
|
Amp+
|
5126
|
|
pcDNA6/V5-His A
|
CMV-Profor(777bp)/T7(863bp)
|
BGH(1121bp)/V5rev(1048bp)
|
Amp+
|
5148
|
|
pcDNA6/V5-His B
|
CMV-Profor(777bp)/T7(863bp)
|
BGH(1121bp)/V5rev(1048bp)
|
Amp+
|
5148
|
|
pcDNA6/V5-His C
|
CMV-Profor(777bp)/T7(863bp)
|
BGH(1121bp)/V5rev(1048bp)
|
Amp+
|
5148
|
|
pcDNAII
|
T7(2997bp)
|
SP6(152bp)
|
|
3013
|
|
pcDNA™6.2-GW/EmGFP-miR
|
miRNA-F
|
miRNA-R
|
Amp+
|
5699
|
|
pCEP4
|
EBVrev(367bp)
|
pCEP-F(500bp)
|
Amp+
|
10410
|
|
pCI(neo)
|
17Base(996bp)/T7(1067bp)(17Base在T7之前) pCI-neoF(886bp)
|
EBVrev(1206bp)/T3(1158bp)
|
Amp+
|
5472
|
|
pCMS-EGFP
|
T7(1067bp)
|
T3(1158bp)
|
Amp+
|
5541
|
|
pCMV-3Tag-4A
|
T3(621bp)/PFLAG-CMV-F(536bp)
|
T7(906bp)/BGH(952bp)
|
|
4233
|
|
pCMV5
|
CMV-F(853bp)/PCEP-F(897bp)
|
无
|
Amp+
|
4657
|
|
pCMV5-Flag
|
pCMV5F
|
pCMV5R
|
Amp+
|
|
|
PCMV6
|
V1.5(815bp)/CMV-Profor(861bp)
|
XL39(1077bp)/HGHrev(1022bp)
|
Amp+
|
4665
|
|
PCMV6-XL4
|
CMV-F(882bp)/T7(952bp)/SP6(952bp)
|
M13R(1048bp)/HGHrev(1089bp)
|
Amp+
|
4707
|
|
PCMV-AC
|
V1.5
|
XL39(同CMV-24)
|
|
5939
|
|
pCMV-HA
|
CMV-Profor(485bp)/TREfor(570bp)
|
EBVrev(965bp)
|
Amp+
|
3782
|
|
pCMV-MCS
|
F(β-globin)
|
R(hGH polyA)
|
|
4492
|
|
pCMV-MYC
|
pCMV-F(794bp)/CMV-Profor(490bp)/TREfor(570bp)
|
pCMV-R(965bp)/EBVrev(973bp)
|
Amp+
|
3791
|
|
pCMV-Sport1
|
T7(646bp)
|
SP6(806bp)/pLNCX-F(924)/CMV-F(970bp)(优先用SP6)
|
Amp+
|
4380
|
|
pCMV-Sport6
|
M13F(663bp)/T7(691bp)
|
SP6(872bp)/M13R(899bp)
|
|
4397
|
|
pCMV-Tag(KAN+)
|
T3(621bp)
|
T7(840bp)
|
Amp+
|
4219
|
|
pCMV-Tag1(KAN+)
|
T3(621bp)
|
T7(840bp)
|
|
4219
|
|
pCMV-Tag2(KAN+)
|
T3(621bp)
|
T7(840bp)
|
|
4219
|
|
pCMV-Tag-2B
|
T3(621bp)
|
T7(845bp)
|
|
4323
|
|
pCMV-Tag3(KAN+)
|
T3(621bp)
|
T7(847bp)
|
|
4219
|
|
pCMV-Tag-3B
|
T3(621bp)
|
T7(846bp)
|
|
4300
|
|
pCMV-Tag4(KAN+)
|
T3(621bp)
|
T7(840bp)
|
|
4219
|
|
pCMV-Tag5(KAN+)
|
T3(621bp)
|
T7(843bp)
|
|
4219
|
|
PCMX-GAL4(MCS区)
|
CMV-F
|
CMV-R
|
|
4500
|
|
pCOLADuet-1(MCS2)
|
T7(214bp)/DuetUP2(189bp)(优先用T7)
|
T7ter(467bp)
|
Kan+
|
3719
|
|
pcoldⅡ
|
pcoldI-F(208bp)
|
pcoldI-R(387bp)
|
|
4392
|
|
pcoldⅢ
|
pcoldI-F(208bp)
|
pcoldI-R(374bp)
|
|
4377
|
|
pcoldⅣ
|
pcoldI-F(208bp)
|
pcoldI-R(356bp)
|
|
4377
|
|
pcoldI
|
pcoldI-F(208bp)
|
pcoldI-R(404bp)
|
|
4407
|
|
pcoldI
|
pcoldI-F(208bp)
|
pcoldI-R(404bp)
|
|
4407
|
|
pcoldIII
|
pcoldI-F
|
pcoldI-R
|
|
4751
|
|
pCold-SUMO
|
pCold-SUMO-F
|
pCold-SUMO-R
|
Amp+
|
4751
|
|
pCold-TF
|
pCold-TF-F1
|
pCold-SUMO-R
|
Amp+
|
5769
|
|
pCR blunt II TOPO
|
SP6(239bp)/M13R(205bp)/RV-M(186bp)
|
T7(425bp)/M13F(449bp)/M13-47(475bp)
|
Amp+/Kan+
|
3512
|
|
pCR2.1
|
M13R(205bp)
|
M13F(406bp，)/T7(382bp)
|
|
3907
|
|
pCR2.1-TOPO
|
M13R(205bp)
|
M13F(409bp)/T7(385bp)(T7离MCS只有6bp，优先用M13F)
|
Amp+/Kan+
|
3909
|
|
pCR3.1
|
T7(638bp)
|
BGH(831bp)
|
Amp+/Kan+
|
5060
|
|
pCR3.1-Uni
|
T7(638bp)
|
BGH(790bp)
|
|
5020
|
|
pCR4-TOPO
|
T3(243bp)/M13R(205bp)/RV-M(186bp)
|
T7(374bp)/M13F(371bp)/M13-47(397bp)
|
Amp+/Kan+
|
3956
|
|
PCRE-LUC
|
RVP3(4976bp)
|
GLP2(327bp)
|
|
5034
|
|
pCR-ScriptSK
|
T7/M13F
|
T3/M13R
|
Amp+/Kan+
|
|
|
pCS2+ (MCSI)
|
SP6(35bp)
|
EBVrev(188bp)
|
Amp+
|
4095
|
|
pCS2+ (MCSII)
|
pSG5rv(222bp)
|
T3(409bp)
|
Amp+
|
4095
|
|
pDBLEU
|
pDBLeu-F(6137bp)
|
pDBLeu-R(6395bp)
|
|
9903
|
|
pDEST15 Gateway
|
T7(25bp)/PGEX-F(721bp)/pGEXrv(5270bp)
|
T7ter(2579bp)
|
Amp+
|
7013
|
|
PDEST20
|
PH-Profor(43bp)/PGEX-F(777bp)/EBVrev(2771bp)
|
无
|
Amp+
|
7066
|
|
PDEST22
|
PDEST22-F(4610bp)/pDEST22-F1998(4540bp)
|
PDEST22-R(6539bp)/pDEST22-R4000(6627bp)
|
Amp+
|
8923
|
|
pDNR-LIB(MCSA)
|
M13F(4065bp)/T7(4089bp)
|
M13R(385bp)
|
|
4200
|
|
pDNR-LIB(MCSB)
|
EBVrev(253bp)
|
M13R(385bp)
|
|
4200
|
|
pDONR/Zeo
|
M13F(536bp)
|
M13R(3044bp)
|
|
4291
|
|
pDONR201
|
SeqL-A(286bp)
|
SeqL-B(2789bp)
|
|
4470
|
|
pDONR207
|
SeqL-A(5567bp)
|
SeqL-B(2485bp)
|
|
5585
|
|
pDONR221
|
M13F(536bp)
|
M13R(3044bp)
|
|
4762
|
|
pDrive
|
T7(239bp)/M13R(204bp)
|
SP6(418bp)/M13F(449bp)
|
Amp+/Kan+
|
3851
|
|
pDsRED1-C1
|
pDsRED-ex-C1-F(1222bp)
|
无
|
|
4686
|
|
pDSRED1-N1
|
CMV-Profor(519bp)
|
RFP-Nrev(751bp)
|
|
4692
|
|
pDsRED2-C1
|
pDsRED-ex-C1-F(1219bp)
|
无反向引物
|
|
4675
|
|
pDSRED-N1
|
pEGFP-N-5'
|
pDSRED-N1-R
|
Kan+
|
|
|
pEarlygate102
|
35S(2580bp)
|
|
|
12453
|
|
PEarlygate201
|
35S(2580bp)
|
|
|
11778
|
|
pEasy-blunt
|
M13R(205bp)
|
M13F(406bp)/T7(382bp)
|
|
3929
|
|
pEasy-E1
|
T7(209bp)
|
T7ter(440bp)(离MCS不到20bp)
|
|
5715
|
|
pEasy-E2
|
T7(5117bp)
|
T7ter(5327bp)
|
|
5393
|
|
pEasy-T1
|
M13R(205bp)/RV-M(186bp)
|
M13F(405bp)/M13-47(431bp)(优先用RV-M和M13-47)
|
|
3928
|
|
pEasy-T1 Simple
|
M13R(205bp)/RV-M(186bp)
|
M13F(306bp)/M13-47(332bp)(优先用RV-M和M13-47)
|
|
3829
|
|
pEasy-T3
|
M13F(2996bp)/T7(3036bp删几个碱基后才能找到，最好不用)
|
M13R(214bp)/SP6(180bp)(优先用M13引物)
|
|
3036
|
|
pECFP-C1
|
EGFP-Cfor(1219bp)
|
SV40-pArev(1576bp)
|
|
4731
|
|
pECFP-N1
|
CMV-Profor(524bp)/pEGFP-N-5’(545bp)
|
pEGFP-N-3’(同EGFP-Nrev)(745bp)
|
|
4733
|
|
pEF/myc/cyto/ER/mito/nuc
|
PEF-F(同EF-1aForward)(1133bp)
|
pCDNA3.1R(1483bp)/c-mycrev(1405bp)
|
|
5603
|
|
pEF/myc/ER
|
PEF-F(同EF-1aForward)(1133bp)
|
BGH(1483bp)
|
Amp+
|
5603
|
|
pEGFP(3'MCS)
|
pEGFP-C-5’(942bp)
|
无反向引物
|
|
3355
|
|
pEGFP(5'MCS)
|
M13R(距第一个MCS位点29bp)(205bp)
|
pEGFP-N-3’(同EGFP-Nrev)(355bp)
|
|
3355
|
|
pEGFP-C1
|
EGFP-Cfor(1219bp)/pEGFP-C-5(1267bp，离MCS较近，不建议使用)
|
SV40-pArev(1575bp)/pEGFP-C-3(1410bp，离MCS较近，不建议使用)
|
|
4731
|
|
pEGFP-C2
|
EGFP-Cfor(1219bp)/pEGFP-C-5(1267bp离MCS较近，不建议使用)
|
SV40-pArev(1579bp)/pEGFP-C-3(1434bp，离MCS较近，不建议使用)
|
|
4735
|
|
pEGFP-C3
|
EGFP-Cfor(1239bp)/pEGFP-C-5(1265bp，离MCS较近，不建议使用)
|
SV40-pArev(1571bp)/pEGFP-C-3(1425bp，离MCS较近，不建议使用)
|
|
4727
|
|
pEGFP-F
|
EGFP-Cfor(1219bp)
|
SV40-pArev(1650bp)
|
|
4806
|
|
pEGFP-N1
|
CMV-Profor(519bp)/pEGFP-N-5'(566bp)
|
pEGFP-N-3’(同EGFP-Nrev)(745bp)
|
|
4733
|
|
pEGFP-N2
|
CMV-Profor(524bp)
|
pEGFP-N-3’(同EGFP-Nrev)(749bp)
|
|
4737
|
|
pEGFP-N3
|
CMV-Profor(524bp)
|
pEGFP-N-3’(同EGFP-Nrev)(741bp)
|
|
4729
|
|
pENTR/D (-TOPO)
|
M13F(536bp)/SeqL-A(481bp)
|
M13R(861bp)/SeqL-B(894bp)
|
Kan+
|
2580
|
|
pET-*(His)
|
T7(4010bp)
|
T7Ter(4191p)
|
Kan+
|
4636
|
|
pET21a
|
T7(5117bp)
|
T7Ter(5357bp)
|
|
5443
|
|
pET22a(AMP+)
|
T7
|
T7Ter
|
|
5493
|
|
pET22b(AMP+)
|
T7(5117bp)
|
T7Ter(5426bp)
|
|
5493
|
|
pET22c(AMP+)
|
T7
|
T7Ter
|
|
5493
|
|
pET-24(+)
|
T7(4984bp)
|
T7Ter(5169bp)
|
|
|
|
pET-24a(+)
|
T7
|
T7Ter
|
|
5236
|
|
pET-24b(+)
|
T7
|
T7Ter
|
|
5236
|
|
pET-24c(+)
|
T7
|
T7Ter
|
|
5236
|
|
pET-24d(+)
|
T7
|
T7Ter
|
|
5236
|
|
pET28a(+)
|
T7
|
T7Ter
|
|
5369
|
|
pET28b(+)
|
T7
|
T7Ter
|
|
5369
|
|
pET28c(+)
|
T7
|
T7Ter
|
|
5369
|
|
pET-30_Ek/LIC
|
T7
|
T7Ter
|
Amp+
|
5439
|
|
pET-30_Xa/LIC
|
T7
|
T7Ter
|
Amp+
|
5448
|
|
pET-30a(+)
|
T7(4988bp)
|
T7ter(5355bp)
|
|
5422
|
|
pET-30b(+)
|
T7
|
T7Ter
|
|
5422
|
|
pET-30c(+)
|
T7
|
T7Ter
|
|
|
|
pET-32a
|
S.tag(5631bp)
|
T7ter(5833bp)
|
|
5900
|
|
pET-32b
|
S.tag
|
T7ter
|
|
5900
|
|
pET-32c
|
S.tag
|
T7ter
|
|
5900
|
|
pET-33b(+)
|
T7(4984bp)
|
T7ter(5316bp)
|
Kan+
|
5383
|
|
pET-34b(+)
|
T7
|
T7ter
|
Kan+
|
5918
|
|
pET-3a
|
T7(1bp)
|
T7ter(186bp)
|
Amp+
|
4640
|
|
pET-3b
|
T7(1bp)
|
T7ter(185bp)
|
Amp+
|
4639
|
|
pET-3c
|
T7(1bp)
|
T7ter(184bp)
|
Amp+
|
4638
|
|
pET-3d
|
T7(1bp)
|
T7ter(183bp)
|
Amp+
|
4637
|
|
pET-40b(+)
|
S.tag(5888bp)
|
T7ter(6123bp)
|
Kan+
|
6190
|
|
pET-41a(+)
|
S.tag(5603bp)/PGEX-F(5455bp)
|
T7ter(5866bp)
|
Kan+
|
5933
|
|
pET-41b(+)
|
S.tag(5603bp)/PGEX-F(5455bp)
|
T7ter(5865bp)
|
Kan+
|
5932
|
|
pET-41c(+)
|
S.tag(5603bp)/PGEX-F(5455bp)
|
T7ter(5867bp)
|
Kan+
|
5934
|
|
pET-42a(+)
|
S.tag(5603bp)/PGEX-F(5455bp)
|
T7ter(5863bp)
|
Kan+
|
5930
|
|
pET-42b(+)
|
S.tag(5603bp)/PGEX-F(5455bp)
|
T7ter(5862bp)
|
Kan+
|
5929
|
|
pET-42c(+)
|
S.tag(5603bp)/PGEX-F(5455bp)
|
T7ter(5864bp)
|
Kan+
|
5931
|
|
pET-43.1a(+)
|
无
|
T7ter(7209bp)
|
|
7275
|
|
pET-43.1b(+)
|
无
|
T7ter(7207bp)
|
|
7274
|
|
pET-43.1c(+)
|
无
|
T7ter(7163bp)
|
|
7230
|
|
pET-44_Ek/LIC
|
S.tag/T7(如果S.tag被切除，可以用T7，正常情况下不建议使用T7)
|
T7ter
|
Amp+
|
7331
|
|
pET-44a(+)
|
S.tag(6545bp)/T7(4870bp,如果S.tag被切除，可以用T7，正常情况下不建议使用T7)
|
T7ter(7244bp)
|
Amp+
|
7310
|
|
pET-44b(+)
|
S.tag/T7(如果S.tag被切除，可以用T7，正常情况下不建议使用T7)
|
T7ter
|
Amp+
|
7310
|
|
pET-44c(+)
|
S.tag/T7(如果S.tag被切除，可以用T7，正常情况下不建议使用T7)
|
T7ter
|
Amp+
|
7310
|
|
pET-45b(+)
|
T7(4870bp)
|
T7ter(5193bp)
|
Amp+
|
5260
|
|
pET46-Ek/LIC
|
T7(4870bp)
|
T7ter(5133bp)
|
Amp+
|
5200
|
|
pET49b
|
PGEX-F
|
T7ter
|
|
5926
|
|
pET-49b(+)
|
PGEX-F(5455bp)
|
T7ter(5859bp)
|
|
5926
|
|
pET50b(+)
|
S.tag(6545bp)/T7(4751bp，如果S.tag被切除，可以用T7，正常情况下不建议使用T7)
|
T7ter(6666bp)
|
|
6733
|
|
pET-9a
|
T7(3711bp)
|
T7ter(3896bp)
|
Kan+
|
4341
|
|
pET-9b
|
T7(3711bp)
|
T7ter(3895bp)
|
Kan+
|
4340
|
|
pET-9c
|
T7(3711bp)
|
T7ter(3894bp)
|
Kan+
|
4339
|
|
pETDuet-1(MCSI)
|
pBRrevBam(5297bp)
|
DuetDOWN1(208bp)
|
Amp+
|
5420
|
|
pETDuet-1(MCSII)
|
DuetUP2(189bp)/T7(214bp)
|
T7ter(467bp)
|
Amp+
|
5420
|
|
pEYFP-C1
|
pEGFP-C-5’(1266bp)
|
pEGFP-C-3'(1429bp)/SV40-pArev(1575bp)
|
Kan+
|
4731
|
|
pEYFP-N1
|
pEGFP-N-5’(545bp)/CMV-Profor(519bp)
|
pEGFP-N-3’(同SV40-pArev)(745bp)
|
Kan+
|
4733
|
|
pFastBac Dual (MCSI)
|
BaculoVfor(4531bp)/PH-Profor(4494bp)
|
FastBac-rev(4753bp)/SV40-pArev(4884bp)
|
|
5238
|
|
pFastBac Dual (MCSII)
|
HSVtk-pArev(4236bp)
|
p10-Profor(4456bp)
|
|
5238
|
|
pFastBac HT_A
|
pFastBac-fw(4040bp)/PH-Profor(3920bp)/BaculoVfor(3957bp)
|
pFastBac-rev(4271bp)
|
|
4856
|
|
pFastBac HT_B
|
pFastBac-fw(4040bp)/PH-Profor(3920bp)/BaculoVfor(3957bp)
|
pFastBac-rev(4271bp)
|
|
4856
|
|
pFastBac HT_C
|
pFastBac-fw(4040bp)/PH-Profor(3920bp)/BaculoVfor(3957bp)
|
pFastBac-rev(4271bp)
|
|
4856
|
|
pFastBac1
|
PH-Profor(3920bp)/BaculoVfor(3957bp)
|
pFastBac-rev(4191bp)
|
|
4776
|
|
pFastBac1-Gus
|
无正向引物
|
SV40-pArev(6208bp)
|
|
6661
|
|
pFastBacHT-CAT
|
无正向引物
|
SV40-pArev(5046bp)
|
|
5500
|
|
pFLAG-ATS
|
N26for(18bp)
|
pTrcHis-rv(同pBADrv)(285bp)
|
|
5408
|
|
pFLAG-CMV1
|
pFLAG-CMV-F(863bp)/CMV-30(834bp)
|
pFLAG-CMV-R(1125bp)/CMV-24(1144bp)
|
Amp+
|
4732
|
|
pFLAG-CMV2
|
pFLAG-CMV-F(863bp)/CMV-30(834bp)
|
pFLAG-CMV-R(1083bp)/CMV-24(1102bp)
|
Amp+
|
4679
|
|
pFLAG-MAC
|
N26for(18bp)
|
pTrcHis-rv(同pBADrv)(225bp)
|
|
5063
|
|
pGAD424
|
GAL4AD(5'AD)(757bp)
|
3'AD(937bp)/pGAL4-Adrv(900bp)
|
Amp+
|
6659
|
|
pGADGH
|
GAL4AD(5'AD)(748bp)
|
T7(933bp)/3'AD(1045bp)
|
Amp+
|
8009
|
|
pGADT7
|
5'AD(1827bp)/T7(1905bp)
|
pGAL4-Adrv(2075bp)
|
|
7988
|
|
pGADT7-Rec
|
5'AD(1858bp)/T7(1905bp)
|
3'AD(2154bp)
|
Amp+
|
8059
|
|
pGAPZa_A
|
a-FACTOR(同Alphafor)(696bp)/GAP-DH-Profor(455bp)
|
3'AOX(976bp)/c-mycrev(850bp)/AOX1rev(994bp)
|
Kan+
|
3147
|
|
pGAPZa_B
|
a-FACTOR(同Alphafor)(696bp)/GAP-DH-Profor(455bp)
|
3'AOX(976bp)/c-mycrev(850bp)/AOX1rev(994bp)
|
|
3147
|
|
pGAPZa_C
|
a-FACTOR(同Alphafor)(696bp)/GAP-DH-Profor(455bp)
|
3'AOX(976bp)/c-mycrev(850bp)/AOX1rev(994bp)
|
|
3147
|
|
pGBKT7(KAN+)
|
T7(1213bp)/GAL4-BD-Cfor(1155bp)
|
3'BD(1494bp)/pGAL4-BDrv(1429bp)
|
Amp+
|
7304
|
|
pGEM-T(Easy)
|
M13F(2975bp)/T7(2999bp)
|
SP6(158bp)/M13R(192bp)
|
Amp+
|
3015
|
|
pGene/V5-His A (GeneSwitch)
|
pLNCX-F(190bp)/Pgene-F(357bp)
|
BGH(659bp)/v5rev(586bp)
|
Amp+
|
4650
|
|
pGene/V5-His B (GeneSwitch)
|
pLNCX-F(190bp)/Pgene-F(357bp)
|
BGH(663bp)/v5rev(590bp)
|
Amp+
|
4654
|
|
pGene/V5-His C (GeneSwitch)
|
pLNCX-F(190bp)/Pgene-F(357bp)
|
BGH(655bp)/v5rev(582bp)
|
Amp+
|
4646
|
|
pGEX-2T
|
PGEX-F(874bp)
|
PGEX-R(1016bp)
|
|
4948
|
|
pGEX-2TK
|
PGEX-F(874bp)
|
PGEX-R(1037bp)
|
|
4969
|
|
pGEX-3X
|
PGEX-F(874bp)
|
PGEX-R(1002bp)
|
|
4952
|
|
pGEX-4T-1
|
PGEX-F
|
PGEX-R
|
|
4969
|
|
pGEX-4T-2
|
PGEX-F
|
PGEX-R
|
|
4970
|
|
pGEX-4T-3
|
PGEX-F
|
PGEX-R
|
|
4968
|
|
pGEX-5X-1
|
PGEX-F
|
PGEX-R
|
|
4972
|
|
pGEX-5X-2
|
PGEX-F
|
PGEX-R
|
|
4973
|
|
pGEX-5X-3
|
PGEX-F
|
PGEX-R
|
|
4974
|
|
pGEX-6P-1
|
PGEX-F
|
PGEX-R
|
|
4984
|
|
pGEX-6P-2
|
PGEX-F
|
PGEX-R
|
|
4985
|
|
pGEX-6P-3
|
PGEX-F
|
PGEX-R
|
|
4983
|
|
pGEX-KG
|
PGEX-F
|
PGEX-R
|
|
5006
|
|
pGL2-Basic
|
GLP1(5564bp)
|
GLP2(99bp)
|
|
5598
|
|
pGL2-Control
|
GLP1(6013bp)
|
GLP2(291bp)
|
|
6046
|
|
pGL2-Enhancer
|
GLP1(5821bp)
|
GLP2(99bp)
|
|
5854
|
|
pGL2-Promotor
|
GLP1(5756bp)
|
GLP2(291bp)
|
|
5789
|
|
pGL3-Basic (MCS I)
|
RVP3(4760bp)
|
GLP2(111bp)
|
|
4818
|
|
pGL3-Basic (MCS II)
|
无
|
RVP4(2080bp)
|
|
4818
|
|
pGL3-Control (MCS I)
|
RVP3(5198bp)
|
GLP2(303bp)
|
|
5256
|
|
pGL3-Control (MCS II)
|
pLXSNrv(2407bp)
|
RVP4(2518bp)
|
|
5256
|
|
pGL3-Enhancer (MCS I)
|
RVP3(5006bp)
|
GLP2(111bp)
|
|
5064
|
|
pGL3-Enhancer (MCS II)
|
pLXSNrv(2215bp)
|
RVP4(2326bp)
|
|
5064
|
|
pGL3-Promoter (MCS I)
|
RVP3(4952bp)
|
GLP2(303bp)
|
|
5010
|
|
pGL3-Promoter (MCS II)
|
无
|
RVP4(2272bp)
|
|
5010
|
|
PGL4.10
|
RVP3(4191bp)
|
GLP2(130bp)
|
|
4242
|
|
pGL4.70
|
RVP3(3471bp)
|
hrRLuc-Nrev(148bp)
|
Amp+
|
3522
|
|
pGS21a
|
T7(1bp)
|
T7ter(982bp)
|
|
6169
|
|
pHEN2
|
M13R(2206bp)
|
无
|
Amp+
|
4616
|
|
pinpointTM
|
PinpointPrimer(325bp)
|
SP6(468bp)
|
|
3331
|
|
pIRES (MCS I)
|
T7 (1067bp)
|
pIRESrv(1125bp)
|
Amp+
|
6092
|
|
pIRES (MCS II)
|
无
|
T3(1776bp)/EBVrev(1824bp)
|
Kan+
|
6092
|
|
pIRES2-EGFP
|
pIRES2-EGFP.P5(531bp)/CMV-Profor(524bp)
|
pIRES2-EGFP.P3(732bp)/IRES-R(781bp)
|
Amp+
|
5308
|
|
pIRES-hrGFP-2a
|
CMV-Profor(530bp)/ T3(521bp)
|
无
|
Amp+
|
4988
|
|
pIRESneo2
|
T7(863bp)/CMV-F(769bp)
|
pIRESrv(1326bp)
|
Amp+
|
5295
|
|
pIVEX 2.3d
|
T7(620bp)/pBRrevBam(517bp)
|
T7ter(882bp)
|
Amp+
|
3560
|
|
pJET1.2/blunt
|
PJET1.2-F(310bp)/T7(305bp)
|
PJET1.2-R(429bp)
|
Amp+
|
2974
|
|
pJG4-5
|
pJG4-5Forward(807)
|
pJG4-5Reverse(906)
|
|
6449
|
|
pkk223
|
pKKrv(4534bp)/pBADrv(4512bp)
|
pKKfw(31bp)
|
|
4586
|
|
pkk223-3
|
pKKrv(4534bp)/pBADrv(4512bp)
|
pKKfw(31bp)
|
|
4586
|
|
pLEGFP-C1
|
EGFP-Cfor(3680bp)
|
pLNCX-R(3960bp)
|
|
6892
|
|
pLenti6/V5 D-TOPO Gateway (ViraPower)
|
CMV-F(2274bp)/PCEP-F(2318bp)/pLNCX-F(2320bp)
|
v5rev(2508bp)
|
|
6963
|
|
pLex
|
无
|
M13F(2507bp)
|
Amp+
|
2886
|
|
pLEXA
|
PLEXA-F(2081bp)/LexAfor (2081bp)
|
PLEXA-R(2298bp)/pGAL4-BDrv (2225bp)
|
|
10166
|
|
pLKO.1-TRC
|
SV40(1839bp)/EBVrev(1652bp)
|
T7(1878bp)/M13F(1923bp)/M13-47(1949bp)
|
|
8901
|
|
PLL3.7
|
U6-promoter-ZL(2476bp)
|
|
|
7650
|
|
pLNCX
|
PLNCX-F(804bp)
|
PLNCX-R(3544bp)
|
|
6620
|
|
pLNCX2
|
CMV-Profor(2841bp)
|
pRevTRErv(3033bp)
|
|
6133
|
|
PLVX-SHRNA1
|
U6-promoter(human)(2383bp)
|
无
|
Amp+
|
7758
|
|
pLXSN
|
PLXSN-F(1574bp)/pLXSNfw (1572bp)
|
PLXSN-R(1759bp)/pLXSNrv(1701bp)
|
|
5874
|
|
pMAL-c2E
|
malE(2587bp)
|
M13-47(2782bp)
|
Amp+
|
6649
|
|
pMAL-C2x
|
pMAL-C2x-F(2558bp)
|
pMAL-C2x-R(2803bp)
|
Amp+
|
6646
|
|
pMAL-C5x
|
malE(2587bp)
|
pMAL-C5x-R(2846bp)
|
|
5677
|
|
pMAL-p2X
|
malE(2662bp)
|
M13-47(2854bp)
|
Amp+
|
6721
|
|
PMCSG7
|
T7ter(68bp)
|
T7(376bp)
|
|
5286
|
|
pMD18-T
|
RV-M (484bp)
|
M13-47(375bp)
|
Amp+
|
2692
|
|
pMD19-T
|
M13-47(352bp)
|
RV-M (507bp)
|
Amp+
|
2692
|
|
PMD20-T
|
RV-M(2707bp)
|
M13-47(170bp)
|
|
2737
|
|
pMSCV-hyg
|
pLXSNfw(1332bp)
|
pMSCVrev(1672bp)
|
Amp+
|
6971
|
|
pMSCV-neo
|
pLXSNfw(1332bp)
|
pMSCVrev(1679bp)
|
Amp+
|
6467
|
|
pMSCV-puro
|
pLXSNfw(1332bp)
|
pMSCVrev(1659bp)
|
Amp+
|
6295
|
|
pMT/V5-HisA
|
MT-Profor(814bp)
|
BGH(1038bp)/v5rev(965bp)
|
|
3538
|
|
pMT/V6-HisB
|
MT-Profor
|
BGH/v5rev
|
|
|
|
pMT/V7-HisC
|
MT-Profor
|
BGH/v5rev
|
|
|
|
pOTB7
|
M13F(110bp)/SP6(53bp)
|
M13R(252bp)/T7(311bp)
|
|
1815
|
|
pPCR-Script-Amp
|
M13-47(573bp)/M13F(599bp)/T7(626bp)
|
T3(790bp)/M13R(828bp)/RV-M(847bp)
|
|
2961
|
|
pPCR-Script-CAM
|
M13-47(573bp)/M13F(599bp)T7(626bp)
|
T3(790bp)/M13R(828bp)/RV-M(847bp)
|
|
3399
|
|
pPIC9K(AMP+、ZEO+)
|
a-FACTOR(同Alphafor)(1152bp)/5’AOX(855bp,离MCS较远，优先用a-factor)
|
3'AOX(1348bp)
|
Amp+
|
9300
|
|
pPICZa
|
5'AOX(同AOX1for)(854bp)
|
3'AOX(同AOX1rev)(1180bp)/C-mycrev(1035bp)
|
Amp+
|
3329
|
|
pPICZb
|
5'AOX(同AOX1for)
|
3'AOX(同AOX1rev)/C-mycrev
|
Amp+
|
3329
|
|
pPICZc
|
5'AOX(同AOX1for)
|
3'AOX(同AOX1rev)/C-mycrev
|
Amp+
|
3329
|
|
pPROEXHTa
|
RV-M(234bp)
|
pBADrv(同pTrcHis-rv)(483bp)
|
|
4751
|
|
pPROEXHTb
|
RV-M
|
pBADrv(同pTrcHis-rv)
|
|
4751
|
|
pPROEXHTc
|
RV-M
|
pBADrv(同pTrcHis-rv)
|
|
4751
|
|
PPZP111
|
M13R(8467bp)/RV-M(8448bp)
|
M13F(8571bp)/M13-47(8597bp)
|
|
8909
|
|
PPZP201
|
M13R(6697bp)/RV-M(6678bp)
|
M13F(6801bp)/M13-47(6827bp)
|
|
7132
|
|
PQCXIN
|
pLNCX-F(2108bp)
|
IRES-R(2404bp)
|
Amp+
|
7381
|
|
pQE30
|
pQE30-F(65bp)/TypeIII/IV(69bp)
|
pQE30-R(243bp)
|
Amp+
|
3461
|
|
pQE40
|
pQE30-F(65bp)
|
pQE30-R(813bp)
|
Amp+
|
4031
|
|
pQE80L
|
BLA-Prorev(4573bp)TypeIII/IV(69bp)pQE30-F(65bp)
|
pQE30-R(243bp)
|
|
4751
|
|
PQE-trisystem
|
TriExup(1021bp)
|
TriExdowm(1529bp)
|
Amp+
|
5766
|
|
pQE-TriSystemHis.Strep 1
|
PCAG-F(1012bp)
|
Baculovirs_rev(1812bp)/BaculoVrev(1931bp)
|
|
5760
|
|
PRK5-flag
|
SP6(828bp)
|
pSG5rv(同SV40-pArev)(1030bp)
|
|
4661
|
|
pRNA-U6.1/Neo
|
T7
|
BGH
|
Amp+
|
|
|
pRS424
|
M13F(1840bp)/T7(1867bp)/pBluescriptKS(1910bp)
|
无
|
|
5616
|
|
pRSET
|
T7(20bp)/Xpressfor(139bp)
|
T7TER(317bp)
|
|
2897
|
|
pRSFDuet-1(MCSI)
|
DuetUP1(3771bp)
|
DuetDOWN1(208bp)
|
|
3829
|
|
pRSFDuet-1(MCSII)
|
DuetUP2(189bp)
|
T7TER(467bp)
|
|
3829
|
|
pSectag(v5/His/a,b,c)
|
T7(863bp)
|
BGH(1154bp)
|
Kan+
|
5185
|
|
pSHUTTLE-CMV
|
CMV-F(857bp)
|
PSHUTTLE-CMV-R(1031bp)/SV40-pArev(同pSG5rv)(1173bp)
|
|
7469
|
|
pSilencer_4.1-CMV_neo
|
Psilencer-4.1-F(377bp)
|
Psilencer-4.1-R(639bp)
|
|
5590
|
|
pSilencer2.0-U6
|
T7(271bp)
|
2.0REV/M13F(696bp)(M13F比2.0REV离MCS近30bp左右)
|
Amp+
|
3075
|
|
pSilencer3.1-H1hygro#
|
M13-47(352bp)
|
3.0REV(1163bp)
|
|
5597
|
|
pSIREN-RetroQ
|
pSIREN-F(6188bp)/PBABE5'(6141bp)/PLXCN/pLXSN-F(6105bp)
|
pMSCVrev(252bp)
|
|
6445
|
|
pSP64
|
SP6(2982bp)/pBRrevBam(2673bp,离MCS稍远，优先用SP6)
|
M13R(83bp)/RV-M(102bp)
|
Amp+
|
2999
|
|
pSP65
|
SP6(2989bp)/pBRrevBam(2679bp,离MCS稍远，优先用SP6)
|
M13R(89bp)/RV-M(108bp)
|
Amp+
|
3005
|
|
pSP72
|
SP6(2446bp)
|
T7(118bp)
|
Amp+
|
2462
|
|
pSport1
|
SP6(165bp)/M13F(139bp)/M13-47(113bp)
|
T7(319bp)/M13R(353bp)/RV-M(372bp)
|
|
4110
|
|
pSTBlue-1
|
T7(24bp)/M13R(3839bp)
|
SP6(202bp)/M13F(233bp)
|
Amp+
|
3851
|
|
PSTV28
|
M13-47(1640bp)
|
RV-M(1789bp)
|
|
2999
|
|
pSUPER
|
T7(626bp)/M13F(599bp)
|
T3(1005bp)/M13R(1043bp)
|
|
3176
|
|
pT-Adv
|
M13R(205bp)
|
M13F(405bp)/T7(381bp)
|
|
3906
|
|
pTargeT™
|
pTarget.F(1181bp)(在T7上游)/T7(1229bp)
|
pTarget.R(1454bp)
|
|
5670
|
|
PTG19-T
|
M13F
|
M13R
|
|
2880
|
|
pThioHis A
|
TrxForward(3777bp)
|
M13F(同TrxReverse/M13F-20)(3986bp)
|
|
4365
|
|
pThioHis B
|
TrxForward(3777bp)
|
M13F(同TrxReverse/M13F-20)(3984bp)
|
|
4363
|
|
pThioHis C
|
TrxForward(3777bp)
|
M13F(同TrxReverse/M13F-20)(3988bp)
|
|
4367
|
|
ptkLUC (MCSI 1-270)
|
EBVrev(4428bp)
|
LucNrev(322bp)
|
Amp+
|
4711
|
|
ptkLUC (MCSII：2100)
|
无
|
M13R(2200bp)
|
Amp+
|
4711
|
|
pTRC99a-c
|
pTRC99C-F(146bp)
|
pTRC99C-R(385bp)
|
|
4176
|
|
pTrcHis A
|
Xpressfor(452bp)
|
无
|
|
4414
|
|
pTrcHis B
|
Xpressfor
|
无
|
|
|
|
pTrcHis C
|
Xpressfor
|
无
|
|
|
|
PTRE(clontech)
|
PCEP-F(289bp)/pLNCX-F(291bp)
|
无
|
|
2605
|
|
pTriEx1.1_Neo
|
T7(2150bp)/TriExup(2099bp)
|
IRES-R(2608bp)
|
|
6664
|
|
pTriPLEx2
|
TriplEx-LD-5´(521bp)
|
T7(680bp)/TriplEx-LDrv (678bp)
|
|
3589
|
|
PTZ57R/T
|
M13F
|
T7/M13R
|
|
2886
|
|
pUC118
|
RV-M(186bp)/M13R(205bp)
|
M13-47(334bp)/M13F(308bp)
|
|
3162
|
|
pUC119
|
RV-M(186bp)/M13R(205bp)
|
M13-47(334bp)/M13F(308bp)
|
|
3162
|
|
pUC18
|
M13-47(352bp)/M13F(378bp)
|
RV-M(500bp)/M13R(481bp)
|
|
2686
|
|
pUC19
|
M13-47(352bp)/M13F(378bp)
|
RV-M(500bp)/M13R(481bp)
|
|
2686
|
|
pUC20
|
M13F
|
M13R
|
|
2705
|
|
pUC57-T
|
M13-47(352bp)/M13F(378bp)
|
RV-M(524bp)/M13R(505bp)
|
Amp+
|
2710
|
|
pUCm-T
|
M13F(378bp)/T7
|
M13R(482bp)
|
Kan+
|
2773
|
|
pUM-T
|
M13F
|
M13R
|
|
2726
|
|
pVAX1
|
T7(664bp)/ CMV-Profor(575bp)
|
BGH(841bp)
|
|
2999
|
|
pwpxl
|
PEF-F/EF-1aForward(3245bp)
|
无反向引物
|
|
10501
|
|
pWSK29
|
T7/M13F/M13-47
|
T3/M13R/RV-M
|
|
|
|
pXT7
|
T7
|
SP6
|
|
|
|
pYES2
|
pYes2.R(同CYC1-Terminator)(218bp)
|
GAL1-Profor(509bp)/T7(386bp)
|
|
5857
|
|
pYES2/CT
|
GAL1-Profor(352bp)/T7(475bp)
|
pYes2.R(同CYC1-Terminator)(743bp)
|
|
5963
|
|
pYES3/CT
|
GAL1-Profor(352bp)/T7(475bp)
|
pYes2.R(同CYC1-Terminator)(743bp)
|
|
5870
|
|
TOPO-PENTR-SD
|
M13F(536bp)
|
M13R(862bp)
|
|
2580
|
编号：54643153464444 备案号：浙ICP备11028186号-1
